Submit a job — v3.0.0-beta6

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Input type

All four accept a FASTA file (protein or nucleotide).

Protein sequencesFASTA · amino acid CDSFASTA · coding nucleotide Genome / contigsFASTA · gene prediction runs first MetagenomeFASTA · mixed community

Nucleotide input is prokaryote-only

CDS, genome/contig and metagenome inputs are supported for prokaryotes only (gene prediction uses Prodigal). Eukaryotic genome annotation is not implemented — for eukaryotes, submit protein sequences.

Input file

A FASTA file of proteins, CDS, or genome contigs — plain text or gzip-compressed (.gz). Max 100,000 sequences.

Drop a FASTA file here, or choose one — validated on upload.

eggNOG-mapper version

v3.0.0-beta6 · recommended eggNOG 7 orthologous groups, per-query taxonomic scoping, curated-only transfer with a lazy closest-cascade. Recommended for new analyses. v2.1.14 eggNOG 5.0 (legacy). DIAMOND search only on this server. Use for reproducing older results.

Basic options

Search mode ?

Gene prediction ?

Genetic code (trans_table) ?

Report orthologs ?

Add MD5 of each query ?

Also produce Excel (.xlsx) ?

Advanced options

DIAMOND sensitivity (ceiling) ?

Taxonomic scope ?

E-value ?

Min % identity ?

Min query coverage ?

Min subject coverage ?

PFAM realign ?

Annotation donors ?

Target orthologs ?

Seed ortholog e-value ?

Target taxa ?

Excluded taxa ?

DIAMOND iterate ?

Report dropped queries ?

Decorate GFF (genomes) ?

Skip annotation (hits only) ?

Override existing outputs ?